生根阶段组培微环境对草莓组培苗生长及光合的影响

Effect of microenvironment on the growth and photosynthesis of tissue-cultured strawberry plantlets at rooting stage

未生根的草莓苗在3%蔗糖或无糖培养基中诱导生根,同时分别给予(60±10)(、150±10)μmol.m-2.s-1两种光照及(350±50)、(700±100)μL.L-1两种CO2浓度,培养20d后测定各项指标。结果表明,草莓组培苗的整体生长势以添加3%蔗糖处理的较好,提高光照和CO2浓度进一步促进其生长。较高光照降低了叶绿素含量,而类胡萝卜素的相对含量有所提高。在未增加CO2的条件下蔗糖对组培苗的光合有轻微的负影响,而提高CO2浓度对组培苗光合速率有积极影响。

The unrooted tissue-cultured plantlets were cultured for 20 days on MS medium containing 0% or 3% sucrose under conditions of（60±10） or（150±10）μmol·m^-2·s^-1light intensity and（350±50） or（700±100） μL·L^-1 CO2 concentration.The results showed that the growth vigor of the plantlets on the MS medium with 3% sucrose was better,and increasing light intensity and CO2 concentration could further promote the growth of plantlets.The higher light intensity reduced the chlorophyll content but increased the relative amount of carotenoid.Adding sucrose without increasing CO2 had a slightly negative effect on the photosynthesis of plantlets,but increasing CO2 concentration could significantly improve the photosynthetic rate of plantlets.