奶牛子宫肥大细胞不同染色方法比较研究

Compare of Mast Cell's Different Staining technique in Cow Uterus

选取四头健康奶牛子宫的子宫角,经10%中性福尔马林固定,对子宫全层进行连续切片。分别应用苏木素-伊红、硫堇、中性红、甲苯胺蓝、阿尔辛蓝-藏红T五种染色法进行染色,来显示肥大细胞。结果表明,甲苯胺蓝染色法显示肥大细胞的效果最佳。

4 healthy cows were taken the part of the uterus horn. The samples were fixed in the 10% neutral formaldehyde solution,and were made into continuous slices of the whole uterine layer. Using Hematoxyln--Eosin, Thioine, Neutral red, Toluidine blue and Alcian blue--safranine T staining technique to stain them to show the mast cell, respectively. The result showed that Toluidine blue staining technique was the best among the five staining techniques.