Egr-1启动子辐射诱导的基因表达特性

The Property of Egr-1 Promoter in Irradiation-Induced Gene Expression

目的:研究电离辐射作用下早期生长反应因子1(Egr-1)启动子调控增强绿色荧光蛋白(EGFP)报告基因在人结肠癌细胞SW480中的表达。方法:利用基因重组手段构建质粒Egr-1-EGFP;阳离子脂质体介导转染人结肠癌细胞SW480,给予不同剂量γ射线照射,流式细胞仪检测照射后不同时间点EGFP的表达。结果:电离辐射能够诱导Egr-1启动子调控下游基因的表达,且6 Gy照射后最为明显,在照射后24 h达到高峰。结论:Egr-1启动子具有电离辐射诱导特性。

Objective： To study the effect of ionizing radiation on the expression of EGFP reporter gene induced by Egr-1 promoter in human colorectal adenocarcinoma cell line SW480. Methods： The plasmid Egr-1-EGFP was constructed by gene recombination, and was transfected into the SW480 cells with cationic liposome, then the cells were treated with various doses of γ-ray irradiation. FACS was used to detect the EGFP expression at different times after exposure. Results： FACS analysis showed that ionizing radiation could induce the expression of EGFP report gene, and this expression reached maximum at 24 h post 6 Gy γ-ray exposure. Conclusion： Egr-1 gene promoter has ionizing radiation-inducible characteristics.