玉米cyclin Ⅲ基因的染色体原位杂交物理定位

The Physical Location of the Maize cyclin Ⅲ Gene by in situ Hybridization

本文首次报道了玉米低拷贝基因ｃｙｃｌｉｎⅢ（Ｂ－类）生物素标记的染色体原位杂交定位结果。供试探针为该基因的ｃＤＮＡ克隆，其长度仅为１６ｋｂ。结果表明，探针的信号分布在第６染色体短臂和第９染色体长臂，与着丝粒的百分距离分别为７００５±３３１和８６８６±１６４，检出率分别为８２９％和６８３％。文中对基因的物理位置与功能间的关系等作了讨论。

A biotin-labelled in situ hybridization technique was used to physically map a low copy gene cyclin Ⅲ in maize. The cDNA clone was 1.7 kb in size. The probe was hybridized onto the short arm of chromosome 6 and the long arm of chromosome 9. The percent distances from centromere to detection site were 70.05±3.31 and 86.86 ±1.64 respetively. The detection rates of in situ hybridization were 8.29 and 6.83 respectively. The relationship between the position and function of the genes is discussed in this paper.