摘要
对非洲菊的花粉形态进行了扫描电镜观察,对4个品种花粉活力和柱头进行了可授性测定,通过荧光显微镜观察了花粉在柱头的萌发情况,结果表明:非洲菊花粉粒为中等偏小型,具三孔沟。在部分ME3培养基中添加300 g.L-1 PEG1500的培养基比较适宜品种‘N2’花粉萌发,生活力最高达38.3%。多数品种的在刚散粉时期,22~28℃萌发能力强。柱头可授期可达12 d。
Test in vitro of germination indicated that the most optimized culture medium was part of ME3+300 g·L^-1 PEG1500. The high- est germination rate in 'N2'was 38.3%. Effect of different flowering stages on pollen viability was significantly. The best condition for pollination was 22-28 ℃ and fresh pollen appearing. By means of benzidine-hydrogen peroxide reaction and aniline blue fluorescence method, showed that the stigma receptivity could teach to 12 days since flowering and the strongest in the inflorescence expanding.
出处
《上海交通大学学报(农业科学版)》
2008年第1期78-80,90,共4页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
上海市农委重点攻关项目(沪农科攻字2003-2-3)
上海市绿化管理局攻关项目(G061206)
关键词
非洲菊
花粉生活力
柱头可授性
Gerberajamesonii
pollen living ability
stigma receptivity