C-erbB-2在非小细胞肺癌中表达的研究

Study on the expression of c-erbB-2 in non-small cell lung cancer

目的探讨c-erbB-2蛋白在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达及其临床意义。方法应用免疫组织化学SABC法检测65例原发性NSCLC和20例正常肺组织中c-erbB-2蛋白的表达水平。结果20例正常肺组织仅2例阳性表达,阳性率为10.0%。NSCLC组阳性表达率为63.1%,两组比较差异有显著性(P<0.05)。其中鳞癌和腺癌的阳性表达率分别为62.5%和63.6%,无显著差异(P>0.05)。在NSCLC中,c-erbB-2在病理分级Ⅰ+Ⅱ级和Ⅲ级的阳性率分别为57.6%和68.8%;在TNM分期Ⅰ+Ⅱ期和Ⅲ+Ⅳ期的阳性表达率分别为60.0%和66.7%,以上两组间比较差异均无显著性(P>0.05)。有无淋巴结转移组的阳性表达率分别为73.7%和48.1%,两组比较差异有显著性(P<0.05)。结论c-erbB-2阳性表达与NSCLC的发生,淋巴结转移呈显著正相关,高表达者提示可能伴有淋巴结转移,临床已进入晚期。

Objective To study the clinical significance of the expression of c-erbB-2 in patients with non-small cell lung cancer method （SABC） was used to detect the expression level of the c-erbB-2 in 65 NSCLC tissue and 20 normal lung tissue specimens. Results c-erbB-2 was stained mainly in the cytoplasm and/or in the surface of NSCLC cells with positive rate of 63.1%. In normal lung tissue, the positive rate of c-erbB-2 was 10%. There were significant difference（ P 〈 0. 05 ） between the two groups. The positive rate of c-erbB-2 in squamous carcinoma was 62. 5% and the adenecarcinoma 63.6%. No significant difference（P 〉0.05）were found between the two groups. The frequency of c-erbB-2 expression in histological grade Ⅰ＋Ⅱ was 57, 6% and the grade Ⅲ 68. 8%. No significant difference（ P 〉 0, 05 ）were found between the two groups. The positive rate of c-erbB-2 in the stage Ⅰ ＋ Ⅱ was 60% and the stage Ⅲ ＋ Ⅳ 66. 7%. There were no significant difference （P 〉 0. 05 ）between the two groups. In the group with lymph node metastasis and the group without lymph node metastasis, the positive rate of c-erbB-2 expression were 73.7% and 48, 1% respectively. Significant difference （ P 〈 0. 05 ） were found between the two groups. Conclusions c-erbB-2 could be used as markers of malignant potential and prognosis factor of NSCLC.