健康成人诱导痰细胞计数正常值参考范围的研究及意义

Research on the significance of standard of the total and differential cell counts of induced sputum among health adult population

目的研究健康成人诱导痰细胞计数及分布规律。方法随机抽取120例健康成人,分为吸烟组和非吸烟组。以4.5%高渗盐水雾化吸入诱导痰,分类计数各细胞成份。结果诱导痰以巨噬细胞和中性粒细胞为主,有少量嗜酸性粒细胞、淋巴细胞和支气管上皮细胞。吸烟组痰细胞总数及中性粒细胞高于非吸烟组(P>0.05)。只有中性粒细胞和巨噬细胞占非鳞状上皮细胞计数的百分比呈正态分布。结论诱导痰中性粒细胞和巨噬细胞占非鳞状上皮细胞的百分比呈正态分布。女性诱导痰嗜酸性粒细胞高于男性。吸烟组高于非吸烟组。

Objective To examine the total and differential cell counts in induced sputum. Methods A total of 120 healthy aduhs were studied. There were 62 smokers and 58 nonsmokers, who inhaled hypertonic （4.5%） saline. We examined the total and differential cell counts in induced sputum. Results Induced sputum cell counts were characterized by a predominance of macrophages and neutrophils and a paucity of eosinophils , lymphocytes, metachromatic cells, and bronchial epthelial cells. Only the percentage counts of macrophages and neutrophils showed a normal distribution ; the total and differential counts of other ceils did not. The total cell and the neutrophils cell counts were higher in the smoking groups （ P 〉0. 05 ） than in the nonsmoker group. The percentage counts of eosinophils were higher in female （ P 〉 0. 05 ） than in male. Conclusion Only the percentage of sputum macrophages and neutrophils showed a norreal distribution. The percentage counts of eosinophils were higher in female. The total ceil and the neutrophils ceil counts were higher in smoking groups.