内皮细胞脂质过氧化损伤增强主动脉平滑肌细胞表达细胞周期素D

Lipid Peroxidation Injury to Endothelial Cell Enhanced Expression of Cyclin D in Aortic Smooth Muscle Cells

为探讨细胞周期素D在主动脉平滑肌细胞中的表述规律，揭示动脉粥样硬化中平滑肌细胞的增殖机制，在培养的免主动脉平滑肌细胞中分别加入不同程度的内皮细胞损伤后的条件培养基，24h后分别收集各组的平滑肌细胞，进行免疫组织化学染色和细胞核的增殖试验。结果发现，主动脉平滑肌细胞可表达细胞周期素D，其免疫反应产物主要位于细胞核内。内皮细胞损伤后的条件培养基能明显促进平滑肌细胞表达细胞周期素D，同时细胞核增殖实验显示，该条件培养基亦能促进平滑肌细胞的增殖．以上结果提示主动脉平滑肌细胞的增殖与细胞周期素D的表达密切相关，损伤后的内皮细胞可通过促进血管平滑肌细胞表达细胞周期素D而诱导平滑肌细胞增殖。

Aim The purpose of the study is to well understand expression of cyclin D in smooth muscle cells and its association with lipid peroxidation injury to endothelial cellls.Methods The lipid peroxidation injury to cultured endothelial cells was induced by diamide,then diamide stimulating endothelial cell-derived conditioned media were collected. After a 24-hour culture of smooth muscle cells exposed to various epithelial cell derived conditioned media, smooth muscle cells were collected, and used to immunocytochemistry stainning and proliferative test.Results Rabbit smooth muscle cells could express cyclin D, the reactant of immune reaction was mainly located in nuclei. The media conditioned by diamide stimulating endothelial cells induced stronger expression of cyclin D in smooth muscle cells (P<0. 01).Meanwhile the amount of 3H-TdR incorporation into DNA in smooth muscle cells induced by the media was obvious larger than that induced by normal endothelial cell derived conditioned media (P<0. 01).Conclusion The proliferation of smooth muscle cells is related to expression of cyclin D, lipid Peroxidation injury to endothelial cell may induce proliferation of smooth muscle cells through enhancing expression of cyclin D.