水稻条纹病毒胁迫下抗、感病水稻品种胼胝质的沉积

Callose deposition in resistant and susceptible rice varieties under Rice stripe virus stress

为进一步探讨脱落酸(abscisic acid,ABA)在水稻条纹病毒(Rice stripe virus,RSV)与水稻互作中的作用,采用徒手切片法及苯胺蓝荧光染色技术研究RSV胁迫对抗、感水稻品种叶片中胼胝质沉积的影响。在RSV胁迫下,感病水稻叶片组织中胼胝质的荧光强度与健株无显著差异;而抗病水稻叶片组织中胼胝质的荧光强度却较健株明显增强,其中,大维管束中的维管束鞘内层厚壁细胞、木质部和韧皮部以及维管束鞘延伸的厚壁组织、小维管束、表皮细胞以及叶肉细胞均有较强的胼胝质荧光出现;且抗病水稻中的胼胝质荧光强度强于感病水稻。这说明水稻品种的抗性与胼胝质的沉积有关,ABA参与了RSV与水稻的互作,增强了水稻抗RSV的作用。

In order to reveal the role of abscisic acid （ABA） in the interaction between Rice stripe virus （RSV） and rice, an observation had been made on the callose deposition in leaves of resistant rice variety K395-418 and susceptible rice variety Wuyujing No. 3 under RSV stress via freehand sectioning and aniline blue staining. Results showed that callose fluorescences in leaves of Wuyujing No. 3 under RSV stress remained constantly compared with the uninoculated control; however, the leaves of KT95-418 under RSV stress had more callose staining, including large vascular bundles and sclerenchyma cells of vascular bundle sheath, small vascular bundles, epidermal cells and mesophyll cells; and more fluorescences of callose deposition were observed in resistant variety than susceptible variety. It was considered that callose deposition and resistance was positively related, and ABA involved in the interaction of RSV and rice.