小麦赤霉病穗组织中黄色镰刀菌和DON毒素的定量分析

Real-time PCR quantification and deoxynivalenol production of Fusarium culmorum in inoculated head tissues of wheat

为阐明小麦赤霉病穗组织中黄色镰刀菌Fusarium culmorum(简称Fc)与毒素产生之间的关系,分别用TaqMan探针实时定量PCR和ELISA法,对接种4天后的17个小麦品系穗组织中的FcDNA和脱氧雪腐镰刀菌烯醇(DON)含量进行了测定。在Fc DNA定量检测中,TaqMan探针实时PCR技术具有高度的稳定性和可重复性。不同小麦品系的Fc DNA含量差异显著,感病品种比抗病品种高100倍左右;DON含量也不相同,Fc生物量的增加伴随着DON产量的提高,两者之间存在对数关系(y=19758lnx-111230)。菌量较低时,DON含量相对较大;而菌量较高时,DON含量相对偏低。

To elucidate the relationship between fungal biomass and deoxynivalenol （DON） production of Fusarium culmorum （Fc） in Fc-inoculated wheat heads, real-time quantitative polymerase chain reaction （RT-Q-PCR） based on TaqMan technology and enzyme-linked immunosorbent assay （ELISA） were used for Fc DNA quantification and toxin measurement in the head tissues of 17 wheat lines or genotypes 4 days after fungal inoculation. TaqMan-based RT-Q-PCR assay was found to be highly reliable and reproducible to measure Fc DNA. Fc DNA and DON concentrations varied in different wheat varieties or＇genotypes. A positive and logarithm correlation was observed between Fc biomass and DON concentrations in the Fc-inoculated wheat heads （y = 19 7581nx - 111 230）.