亲和病原菌诱导黄瓜组织结构抗病性机制

Structural resistance in cucumber induced by pathogen

炭疽病菌预先接种黄瓜可以诱导其产生对褐斑病的抗性。经炭疽病菌(1.0×106个孢子/mL)诱导48h后,黄瓜对褐斑病的抗性最大可达97.83%。在同种马铃薯葡萄糖液体培养基中培养,这两种病原菌的竞争力差异显著,黄瓜炭疽病菌能够获得更多的营养物质,生长旺盛,而褐斑病菌的生长则受到严重抑制。经苯胺兰染色发现,炭疽病菌诱导后黄瓜叶片中胼胝质的积累量显著增加。此外,黄瓜炭疽病菌与褐斑病菌的侵染方式存在一定的差异,前者可以通过气孔或直接穿透细胞壁侵入寄主,而后者只能通过直接穿透细胞壁侵入寄主。

Colletotrichum lagenarium can induce resistance in cucumber against brown-spot. The effect of resistance can reach 97.83% if the cucumber inoculated by Colletotrichum lagenarium （1.0×10^6 spores/mL） 48 hours. The ability of challenge is different, Colletotrichum lagenarium is stronger and absorbed more in the same grade on nutrition. The accumulation of callose in leaves of induced cucumber increased obviously by toluidine blue. Also, results of study of mechanism show that the way of infection is different between CoUetotrichum lagenarium and Corynespora cassiicola after stained. The invasion of former is through stoma or attack cell wall, directly, but the latter by cell wall only.