肠炎沙门菌PFGE分子分型及耐药性研究

Study on PFGE molecular typing and antimicrobial resistance of Salmonella enteritidis

目的：对本地区及相邻地区2004～2007年分离的肠炎沙门菌菌株的分子分型及耐药性进行分析。方法：采用PFGE进行分子分型,利用药敏卡通过仪器对分离菌株进行药敏检测。结果：5起肠炎沙门菌食物中毒分离的菌株分成3型,其中2型之间存在3条带的差异,菌株间有相近关系。结论：PFGE是一种非常有效的分子分型方法,建立细菌PFGE指纹图谱数据库及PulseNet,对于控制食源性疾病非常重要。

Objective：To study the molecular typing and antimicrobial resistance of Salmonella enteritidis isolated from Nanshang and neighboring region from 2004 to 2007.Methods：Use PFGE to identify the molecular type,use antimicrobial-susceptibility card and instrument to survey antimicrobial resistance.Results：Three types of strains were differentiated,which isolated from 5 foodborne disease outbreaks.There were 3 lanes difference in the gene-typing and the relation was close between Salmonella enteritidis strains.Conclusion：PFGE is an effective method of molecular typing.It is important to establish PFGE finger-printing database and PulseNet in preventing foodborne disease outbreaks.