固相萃取-气相色谱法检测蔬菜中17种残留的有机磷农药

Simultaneous determination of 17 organophosphorous pesticide residues in vegetables and fruits by solid phase extraction(SPE) coupled with gas chromatography(GC)

目的：建立固相萃取-气相色谱法测定蔬菜水果中17种有机磷农药残留量的分析方法。方法：蔬菜水果捣碎经乙腈提取,盐析,浓缩,CARB/NH2柱净化,丙酮溶解定容,用AT-1701中性毛细管柱和FPD检测器测定。结果：相关系数为0.9965-0.9993,17种有机磷农药回收率加标1.0 mg/kg时,为80.5%～98.2%之间,加标10.0 mg/kg时,为90.1%～99.7%之间,变异系数RSD,加标1.0 mg/kg时,为1.75%～6.12%之间,加标10.0 mg/kg时,为2.19%～5.82%之间。结论：实验结果表明,在选定的色谱条件下,采用固相萃取-气相色谱法对蔬菜中17种有机磷农药残留量进行测定。本法线性范围宽,线性良好,重现性强,回收率很高,能满足有机磷农药残留量测定,完全能够达到国家标准方法的要求。

Objective：To establish a method for the simultaneous determination of 17 organophosphorous pesticide residues in vegetables and fruits by solid phase extraction（SPE） coupled with gas chromatography（GC）.Methods：The rensult was determined by FPD neutral detector with AT-1701 capillary column after acetonitrile extraction,salting-out,concentration,CARB/NH2 column purification,and dissolved to constant volume with acetone.Results：The recoveries varied between 80.5%~98.2%,RSD was between 1.75%~6.12% when 1.0 mg/kg standard solution were added in and 90.1%~99.7%,2.19%~5.82% respectively when 10.0 mg/kg standard solution were added in.Conclusion：This method has reliable producitivity and can give a wide linear range with good recovery and RSD.So it is suitable for the analysis of organophosphorous pesticide residues in vegetables and fruits.