正常人、龋病和牙髓炎患者牙髓组织中一氧化氮含量的检测分析

Analysis of nitric oxide in normal ,carious and inflammatory human dental pulps tissue

目的：对正常人、龋病和牙髓炎患者牙髓组织中一氧化氮（Ｎｉｔｒｉｃｏｘｉｄｅ，ＮＯ）含量进行检测，初步探讨ＮＯ在牙髓组织自身修复中的作用。方法：采用荧光分光光度法检测其中亚硝酸根（ＮＯ２－）含量，以间接确定ＮＯ含量。结果：正常牙髓组织中ＮＯ无性别差异（Ｐ＞０．０５），浅龋组与对照组ＮＯ无显著性差异（Ｐ＞０．０５），而深龋伴慢性牙髓炎组ＮＯ含量则显著高于对照组（Ｐ＜０．０５）。结论：牙髓组织中ＮＯ含量的变化与其炎症反应有一定的联系，ＮＯ可能在牙髓炎症和自身修复过程中起到一定的作用。

Aim: To deteimine the nitric oxide levels in normal ,carious and inflammatory human pulps tissue ,and to study the function of NO in pulpal inflammation and healing process. Methods: Using fluorometric method for the determination of nitrite, which stands for the levels of NO. Result: It was found that there was no significant difference in NO of healthy individual dental pulps between male and female( P >0.05),and there was no difference between shallow caries groups and control( P >0.05),however ,NO in pulps of deep caries accompanied pulpitis were significantly higher than that in normal pulp( P <0.05). Conclusions: NO may play a role in pulpal inflammation and healing process.