碱性成纤维细胞生长因子在人牙胚中表达的免疫组化定位

Immunolocalization of basic fibroblast growth factor in development human tooth

目的：观察碱性成纤维细胞生长因子（ｂＦＧＦ）表达与人牙胚发育和分化的关系。方法：采用免疫组化方法观察ｂＦＧＦ在人牙胚发育中的定位。结果：钟状期牙胚中前成釉细胞为ｂＦＧＦ强阳性，星网层可疑；成牙本质细胞为强阳性，牙乳头组织中阳性分布不均，靠近成牙本质细胞的牙乳头细胞为阳性；牙囊细胞阳性，血管内皮细胞呈强阳性；骨组织为阴性。结论：ｂＦＧＦ与成釉细胞、成牙本质细胞的分化和成熟有关。

Aim: To provide a insight into effects of bFGF on morphogenetic and cytogenetic events during human tooth development. Methods: We used immuno-histochemical techniques to examine the spatial pattern of bFGF expression. Results: At the bell stage, immunoreactive bFGF was localized to the preameloblasts. The bFGF was immunolocalized intensely within the preodontoblasts and the papilla lay subjacent to pre-odontoblasts. Intense immunostaining was also found in the dental follicle separating the tooth organ from its bony crypt. Cartilage and bone showed no immunostaining. Conclusions: The expression of bFGF might be related to the state of differentiation and maturity of ameloblasts and odontoblasts, and their secretion of extracellular matrix during human tooth development.