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胰岛素抵抗状态下大鼠心肌细胞葡萄糖转运蛋白4变化的研究 被引量：3

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摘要 目的观察胰岛素抵抗状态下葡萄糖转运蛋白4(GLUT4)在心肌细胞内表达的变化。方法30只Wistar雄性大鼠随机分为实验对照组、高脂饮食组、高脂高糖饮食组,分别给予普通饮食、高脂饮食和高脂高糖饮食喂养8w,以建立胰岛素抵抗大鼠模型。用钳夹技术检测胰岛素抵抗的存在,分别测量大鼠的体重、空腹血糖和葡萄糖摄取率,并用原位杂交的方法检测心肌细胞GLUT4mRNA的染色细胞的阳性率,应用SPSS13.0软件进行统计学分析,比较组间各指标的差异,并进行直线相关分析。结果高脂饮食组和高脂高糖组存在胰岛素抵抗,而对照组未出现胰岛素抵抗;高脂组与高脂高糖组大鼠的葡萄糖摄取率明显低于对照组(P<0.05),而高脂组又高于高脂高糖组(P<0.05);与大鼠体重增加值的相关分析表明,高脂组和高脂高糖组的葡萄糖摄取率与大鼠体重的增加值呈显著的负相关(P<0.01);原位杂交法检测心肌细胞内GLUT4mRNA的染色细胞阳性率,高脂组、高脂高糖组的染色阳性率明显低于对照组(P<0.05),而高脂组和高脂高糖组无统计学差异(P>0.05);两者的直线相关分析显示,高脂组和高脂高糖组的GLUT4mRNA的阳性率与葡萄糖摄取率呈正相关(P<0.01)。结论胰岛素抵抗可致心肌细胞GLUT4mRNA转录水平下降。
作者 黄峥 赵晟
机构地区 辽宁省金秋医院循环内科 中国医科大学盛京医院内分泌科
出处 《中国老年学杂志》 CAS CSCD 北大核心 2008年第5期449-451,共3页 Chinese Journal of Gerontology
关键词 胰岛素抵抗 葡萄糖摄取率 葡萄糖转运蛋白
分类号 R-332 [医药卫生]
  • 相关文献

参考文献11

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共引文献22

同被引文献22

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引证文献3

二级引证文献5

中国老年学杂志
2008年 第5期

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