人胃腺癌阿霉素耐药细胞系 BGC-823/DOX 建立

Establishment and characterization of doxorubicin resistant BGC 823/DOX of human gastric cancer cell line

为研究消化道肿瘤耐药机理，作者采用药物递增的方法，建立了体外人胃腺癌阿霉素耐药细胞系ＢＧＣ－８２３／ＤＯＸ，并进行了染色体组型、倍增时间、细胞周期分布等细胞系基本特征的系列分析以及耐药倍数、交叉耐药和初步的逆转耐药的实验研究。结果表明，ＢＧＣ－８２３／ＤＯＸ染色体为亚三倍体组型，倍增时间、细胞周期分布与亲本细胞有显著差异，阿霉素耐药倍数６．３５倍，与ＶＰ－１６存在交叉耐药现象，异搏定有逆转耐药的作用。作者认为，ＢＧＣ－８２３／ＤＯＸ是比较符合临床消化道肿瘤特点的阿霉素耐药细胞系。

To study drug resistance mechanism of gastrointestinal tumor,doxorubicin resistant cell line of human gastric cancer,we developed BGC 823/DOX, by increasing doses of doxorubicin. We completed chromosomal analysis, double time of cell grow, cell cycle distributions,drug resistance and cross resistance, and studied reversing drug resistance. The results showed that chromosome of BGC 823/DOX was subtriploid karyotype. Cell morphology,double time,cell cycle distributions were different from its parent cells and 6 35 times more resistant to cytotoxic action of doxorubicin, cross resistant to VP 16. Verapamil showed modified effect on doxorubicin resistance. BGC 823/DOX with doxorubicin resistance has many characteristics of gastrointestinal tumors.