复合分子印迹聚合物体系选择性富集蛋白质样品中的溶菌酶

Matrix Molecularly Imprinted Polymer for Enriching Lysozyme in Protein Samples

考察了功能单体与模板蛋白的反应摩尔比、溶液pH值及离子强度对功能单体与模板蛋白之间相互作用的影响,得出制备分子印迹聚合物的最佳条件.在最佳条件下,以溶菌酶(Lyz)为模板分子,丙烯酰胺(AA)和N,N′-亚甲基双丙烯酰胺(BisAA)为聚合基质,二氧化硅为固体制孔剂,制备了复合分子印迹聚丙烯酰胺凝胶,并用平衡吸附实验研究了其吸附性能和识别选择性.研究结果表明,该聚合物对模板蛋白有较高的亲和性、选择性和吸附容量,可以从蛋白质混合溶液中分离富集模板分子.

The effects of the molar ratio of functional monomer to template protein, the pH and the ionic strength of the medium on the interaction between the functional monomers and template protein were primarily investigated from the molecular level by UV spectrophotometry. Then lysozyme（Lyz）-imprinted polyacrylamide gel was prepared to recognize effectively Lyz in protein samples. With acrylamide and N, N＇-methylenediacrylamide as the polymeric matrix and silica as the pore-forming reagent, after template and silica were removed, the surface of gel polymer had a large quantity of well-distributed macropores, which were suited to let the pro- teins pass in and out. The recognition and selectivity of the imprinted polymers were studied with equilibrium adsorption. Selective adsorption experiments of the imprinted gel show that imprinted gel exhibited good recognition capability for Lyz compared with bovine serum albumin or ovalbum as the completive substance.