摘要
目的:建立一种快速检测O9抗原沙门菌的新方法。方法:利用针对沙门菌O9抗原的两株特异性单克隆抗体,建立一种双抗体夹心检测O9抗原沙门菌的胶体金免疫层析试纸条(GICA)。结果:该试纸条对标准肠炎沙门菌LPS的最小检出量为0.15 ng/条,对肠炎沙门菌菌液的最小检出量为2×105cfu/条。蛋品、鸡肉、猪肉的人工污染试验显示,经增菌后免疫层析试纸条可检出1 cfu的肠炎沙门菌,在大肠杆菌与沙门菌比例为1000:1仍不干扰试纸条的检测。共对市场采集的鸡蛋、鸡肉以及猪肉样品577份进行免疫层析试纸的检测,试纸条与经典细菌分离鉴定法相比:敏感度为90.0%,特异性为98.6%。结论:检测O9抗原沙门菌的GICA法快速、特异、灵敏,在重要沙门菌病检测方面有着潜在、广阔的应用前景。
Objective：To develop a quick method for detecting Salmonellae bearing O9 antigen.Methods：A gold immunochromatographic assay（GICA） to detect Salmonellae O9 antigen has been developed by using two different monoclonal antibodies that bound distinct epitopes of Salmonellae O9 antigen.Results：The detection limit for this strip was 0.15 ng Salmonella enteritidis LPS and 2×105 cfu for the strain.The strip could detect 1cfu Salmonella enteritidis after enrichment and E.coli didn′t interfere with the detection even at a ratio of 1000：1 with Salmonella enteritidis.GICA detected 577 samples,Salmonellae O9 antigen was detected by GICA which was in accordance with the classical bacterial separation method,with a sensitivity of 90.0% and a specificity of 98.6%.Conclusion：The GICA is simple,rapid and accurate and can be used in human clinics.
出处
《中国卫生检验杂志》
CAS
2008年第1期42-44,共3页
Chinese Journal of Health Laboratory Technology
关键词
O9抗原沙门菌
胶体金免疫层析法
Salmonella O9 antigen
Gold immunochromatographic assay（GICA）
