保健品中褪黑素测定方法的研究

Study on determination of melatonin in health products

目的：摸索更简单、快速的褪黑素（Melatonin）测定方法。方法：采用高效液相色谱法,利用褪黑素在紫外222 nm处有最大吸收峰的特点,结合超声提取、离心分离对其进行含量检测。结果：在选定的条件下,当溶液中褪黑素浓度范围在0.6~30.0μg/ml时,标准曲线的回归方程为Y=27.00X＋21.35,相关系数r=0.9991,平均回收率为91.7%,相对标准偏差为2.6%。结论：通过简化提取方法,达到了简单、快速、准确测定保健品中褪黑素的目的。

Objective：To establish a convenient and rapid method for determination of melatonin.Methods：The maximal ultraviolet absorption of melatonin was 222 nm.So the content was determined by high performance liquid chromatography combined with ultrasonic extraction and centrifugal separation.Results：Under the selected condition,the regression equation was Y=27.00X＋21.35 and r=0.9991 when the concentration of melatonin was 0.6～30.0 μg/ml.The average recovery was 91.7% and the relative standard deviation 2.6%.Conclusion：The method is simple,rapid and accurate for determination of melatonin in health products.