重组痘苗病毒载体介导的IL-2基因局部转染对小鼠黑色素瘤的治疗作用

IN VIVO GENE THERAPY OF MURINE MELANOMA MEDIATED BY LOCAL TRANSFECTION OF RECOMBINANT VACCINIA VIRUS ENCODING HUMAN IL 2

采用编码人ＩＬ－２基因的重组痘苗病毒载体（ｒＶＶ－ＩＬ－２），以ｉｎｖｉｖｏ模式体内局部转染小鼠黑色素瘤，结果肿瘤生长速度明显减慢，荷瘤小鼠存活期显著延长。体内免疫功能的检测表明，荷瘤小鼠脾细胞的ＮＫ活性及诱导后的ＬＡＫ、ＣＴＬ活性明显高于对照组，表明采用ｒＶＶ－ＩＬ－２载体介导ｉｎｖｉｖｏ的肿瘤基因治疗具有一定的前景。

Treated murine melanoma by local transfection of recombinant vaccinia virus encoding human IL 2(rVV IL 2).It was observed that in vivo rVV IL 2 transfection significantly inhibited the tumor growth and prolonged the survival time of tumor bearing mice.The splenocytes from rVV IL 2 treated mice showed higher cytotoxicity of NK,LAK and CTL in comparison with the controls.These results suggested that the in vivo transfection mediated by rVV IL 2 was potentially feasible for the cancer gene therapy.