摘要
应用提取纯化的抗IBDVIgG免疫BALB/c小鼠,取其脾细胞与SP2/0细胞在PEG作用下融合,应用ELISA法检测筛选,经有限稀释法克隆2次,获得2株(2B6株,5F4株)分泌抗IBDV独特型抗体的杂交瘤细胞株,其能诱生BALB/c小鼠产生高效价的含抗IBDV独特型抗体的腹水。用此独特型抗体分别与福氏完全佐剂、福氏不完全佐剂按1∶1比例乳化制备成抗IBDV独特型抗体疫苗,免疫接种SPF鸡和普通京白公鸡,然后用IBDV野毒株经点眼和滴鼻方式攻毒,保护率分别为8/8、14/14,从而证实抗独特型抗体疫苗有潜在的研究和应用价值。
The idiotype antibodies (Ids) against IBDV were prepared by hybridoma technique. The Ids were generated by fusing SP2/0 mouse myeloma cells and splenocytes from BALB/c mice immunized with the purified anti IBDV IgG in the presence of PEG. The Ids in hybridoma culture supernatant were detected by ELISA, then the positive hybridoma were cloned 2 times by the technique of limiting dilution. Two cell lines (2B 6, 5F 4) of hybridoma secreting Ids against IBDV were established, and the ascites of high titers were produced. The vaccine of anti IBDV Ids were made by emulsifying the Ids with complete or incomplete Freund′s adjuvant, inoculated SPF chicken and common Jingbai chicken, then challenged with undomesticated IBDV. The protective ratio was 8/8, 14/14. The results indicated the Ids vaccine seemed to be of value for further study and application.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1997年第4期350-352,共3页
Chinese Journal of Veterinary Science
关键词
鸡病
IBD
IBDV
抗体
疫苗
免疫接种
IBDV
the cell line of hybridoma
idiotype antibody
vaccine
immunity