牛副伤寒活菌苗的交互免疫及应用

Cross Immunity and Application of Bovine Paratyphus Living Vaccine

用都柏林沙门氏菌弱毒株（ＳＴＭ８００２－５５０）制造的牛副伤寒活菌苗免疫豚鼠和牦犊牛，１５ｄ后用２个最小致死量（ＭＬＤ）的都柏林沙门氏菌原始强毒株（Ｓ８００２）和病牛沙门氏菌强毒株（Ｃ７９－８８）分别进行攻击。结果，经用该苗免疫的豚鼠和牦犊牛，对Ｓ８００２强毒株的攻击具有１２／１２和１４／１４的保护；对Ｃ７９－８８强毒株的攻击产生了１１／１２和１４／１４的保护。在甘肃、青海和四川省的发病牧区共免疫注射牦牛８０．５万头，与未免疫牛群相比副伤寒发病率和死亡率分别下降８５．２７％和９０．０１％。证明该菌苗不仅对由都柏林沙门氏菌所致的副伤寒具有良好的免疫效果，而且对由病牛沙门氏菌所致的副伤寒也具有良好的交叉保护作用。

Guinea pigs and calves were inoculated with bovine paratyphus living vaccine prepared from attenuated S.dublin strain (STM8002 550), then challenged with 2 MLD of the virulent S.dublin strain (S8002) and S.bovismorbificans strain (C79 88) respectively after 15 days of the inoculation. The results showed that 12/12 the inoculated Guinea pigs and 14/14 the calves were protected against S8002; 11/12 the Guinea pigs and 14/14 the calves were protected against C79 88. Inoculation of 805 thousand yaks were made in Gansu, Qinghai and Sichuan province. As compared with controls, the incidence and mortality of bovine paratyphus in yaks reduced by 85.27% and 90.01% respectively demonstrating its fine immunity against paratyphus resulted from not only S.dublin but also S.bovismorbificans.