脑缺血再灌注脑内纹状体区细胞外液谷胱甘肽的动态变化及丹参的影响

Time-course Changes of Extracelluar Fluid (ECF) Glutathione during Cerebral Ischemia and Reperfusion in the Striatum of Gerbils and Effect of Radix Salviae Miltiorrhizae.

采用脑内微透析技术，应用高压液相色谱电化学检测方法（ＨＰＬＣ－ＥＤ），活体动态观察沙土鼠全脑缺血３０分钟，再灌注１２０分钟的细胞外液中的谷胱甘肽（Ｇｌｕｔａｔｈｉｏｎｅ，ＧＳＨ）的变化及丹参对它的影响。结果显示：全脑缺血后，细胞外液ＧＳＨ水平迅速升高（Ｐ＜０．０１），缺血３０分钟达高峰为缺血前的５。８２倍。再灌注后ＧＳＨ水平明显降低，于３０分钟趋于正常。脑缺血前３０分钟给予丹参注射液不影响细胞外液ＧＳＨ水平。表明脑缺血及再灌注期，ＧＳＨ反应性增高。ＧＳＨ作为内源性抗氧化剂及ＮＭＤＡ受体拮抗剂在脑缺血损伤中起重要作用。

The glutathione (GSH) levels in intracerebral microdialysis of the striatum of gerbils with 30 min globe ischemia followed by 120 min reperfusion as well as the effect of Radix Salviae Miltiorrhizae (RSM) on GSH levels were investigated by high performance liquid chromatography with electrochemical detection (HPLC-ED). It was found that ECF GSH levels were significantly increased (5.82 fold) during the first 30 min after globe ischemia and decreased ( P <0.01) after reperfusion (0-60min). RSM adiministration 30 min before globe ischemia had no effect on ECF GSH levels.The tesults suggested that the increase of GSH levels may be a reaction to ischemia reperfusion insult and GSH as an endogenous antioxidant and NMDA receptor antagonist may have protective effect on cerebral ischemic injury .