泌尿生殖道沙眼衣原体感染的临床表现特点与基因型分布

Differences in Clinical Manifestations of Genital Chlamydial Infections Related to Serovars

目的了解沙眼衣原体引起的泌尿生殖道感染临床表现与其分离株的基因型的关系。方法应用聚合酶链反应和限制性片段长度多态性分析,对来自妇产科和皮肤性病门诊的泌尿生殖道感染沙眼衣原体患者的92份标本和来自健康体检者沙眼衣原体检测为阳性的68例标本进行了基因分型,并分析其临床表现特点与基因分型的关系。结果共检出D、E、F、G、H、I、J、K等基因型;检出D、E混合感染3例和D、J混合感染1例。有症状和无症状患者中E型、D型、F型、G型检出均较多;H型只在有症状的患者中检出;J型多在无症状的患者中检出。在不同年龄段中,以31～40岁为最多,但有症状患者和无症状患者之间无统计学差异。结论泌尿生殖道沙眼衣原体感染的临床表现与不同基因型感染有关。

Objective To determine whether certain Chlamydia trachomatis serovars are associated with a symptomatic or an asymptomatic course of infection, C. trachomatis serovar distributions were analyzed in symptomatically and asymptomatically infected persons. Methods C. trachomatis-positive 92 cervical and urethral swabs from symptomatically infected men and women were obtained from several hospital-based departments and 68 urine specimens from asymptomatically infected men and women were obtained from population-based screening programs in Chenzhou city, Hunan. Serovars were determined using PCR-based genotyping,i, e, restriction fragment length polymorphism analysis of the nested-PCR-amplified ompl gene. Results Serovars, D, E, F, G, H, I, J and K had been detected, The most prevalent C. trachomatis serovars, D, E, F and G, showed no association with either a symptomatic or asymptomatic course of infection. The most differences found were the association of serovar J more often in asymptomatically infected women （ P = 0. 009 ）. Furthermore, serovars were associated with ages （ P = 0. 002 ） , which had no signifi- cance with clinical manifestations. Conclusion Chlamydia trachomatis serovars are associated with a symptomatic or an asympto- matic course of infection.