HIV-1 env基因序列分析及其与长期感染不进展现象相关性研究

Sequence Analysis of HIV-1 Env Gene in Infected Long-term-Non-progressor

目的研究HIV-1长期感染不进展现象与HIV-1 env基因变异的关系。方法用巢式聚合酶链反应(nested-PCR)对21例感染HIV-1毒株7年以上者外周血单一核细胞(PBMCs)的核酸样本进行扩增,获得HIV-1膜蛋白(env)基因的核酸片段,对其C2-V3及邻区350-450个核苷酸序列进行测定和分析,所得结果与HIV-1该亚型国际标准株进行比较,分析共享序列及突变序列,制作系统树,计算离散率。结果离散率和系统树分析21个毒株均为HIV-1 B亚型,毒株间基因离散率在2%左右,高于前期研究结果;未见V3环顶端四肽特征性GPGR和GRGQ,也未见前期研究曾经发现的脯氨酸向异亮氨酸的变异现象。结论HIV-1长期感染者体内毒株env基因无规律性突变。

Objective To study the relationship between HIV-1 infected long-term-nonprogressor and env gene mutation. Methods HIV-1 cnv gene was amplified by nested-PCR from 21 peripheral blood mononuclear cells （PBMCs） obtained from HIV-1 infected long-term-nonprogressors. The C2-V3 region （350 -450 bp） of them were sequenced and compared with several reference HIV-1 strains. Results Sequence analysis showed that all of 21 strains were subtype B of HIV-1, and gene divergence was about 2% ,which was more than the results got before. There was no mutation of amino acid Pro turning to amino acid Ile and characteristic polypeptide GPGQ and GPGR occurred before. Conclusion The env gene of the HIV-1 strains screened from longterm-nonprogressors showed no regularity of mutation.