蝎毒对人肝癌细胞Bel-7404凋亡及端粒酶逆转录酶基因的作用

The ffects of scorpion venom on Bel-7404 human liver cancer cell death and hTERTmRNA activity

目的观察蝎毒对人肝癌Bel-7404细胞增殖的影响及对端粒酶逆转录酶基因(hTERTmRNA)活性的影响。方法以人肝癌细胞株Bel-7404为研究对象,以不同浓度的蝎毒处理体外培养的人肝癌Bel-7404细胞72 h,以噻唑蓝(MTT)法测定细胞增殖抑制,采用RT-PCR法检测细胞hTERTmRNA的表达情况。结果MTT结果显示蝎毒抑制Bel-7404细胞增殖、诱导细胞凋亡且抑制有浓度依赖性。同时,蝎毒可抑制hTERTmRNA的表达。结论抑制端粒酶逆转录酶基因(hTERTmRNA)表达而抑制端粒酶的活性、诱导细胞凋亡,可能是蝎毒的抗癌细胞机制之一。

Objective To research the effects of scorpion venom on the multiplication of Bel-7404 human liver cancer cells and activity of hTERTmRNA. Methods The depressive effects of different concentrations of scorpion venom solution on the Bel-7404 liver cancer cells which were cultured in vitro were tested over 72 hours with MTT and the expression of hTERTmRNA was tested by RT-PCR. Results MTr results showed scorpion venom could depress the proliferation and induce death of Bel -7404 human liver cancer cells, which was related to the concentration of the scorpion venom solution. Also,scorpion venom could depress the expression of hTERTmRNA. Conclusion scorpion venom can restrain the activity of hTERTm and induce death of cancer cells by inhibiting the expression of hTERTmRNA, it may be one of the anti-cancer mechanisms of scorpion venom.