TLR2在大鼠急性坏死性胰腺炎中末端回肠的表达及意义

Expression of Toll-like receptor 2 in distal ileum of rats with acute necrotizing pancreatitis and its significance

目的:研究大鼠急性坏死性胰腺炎(ANP)末端回肠组织中Toll样受体2(TLR2)的表达及意义.方法:60只大鼠随机分为2组:假手术组20只,ANP组40只.采用逆行胰胆管注射5%牛磺胆酸钠制作大鼠ANP模型,并又分为8 h和16 h组.应用实时定量PCR检测不同组、不同时点末端回肠组织TLR2 mRNA的表达,应用Western blot和免疫组化分析各组TLR2蛋白表达变化及组织学定位.结果:与假手术组相比,ANP时末端回肠组织TLR2 mRNA及蛋白表达显著升高,TLR2的表达水平分别与肠黏膜的病理学评分(r=0.42,P<0.01)和肠黏膜通透性(r=0.41,P<0.01)呈正相关.免疫组化发现,ANP组的回肠黏膜表面、黏膜固有层的T、B淋巴细胞、单核细胞、中性粒细胞、嗜酸性细胞、黏膜下层的动静脉、纵形和环形肌层等处都有比较强的TLR2的表达.结论:ANP时末端回肠组织内TLR7 mRNA和蛋白表达上调,可能与ANP肠黏膜的病理改变和肠源性感染的发生与发展有关.

AIM： To investigate the expression of Toll-like receptor 2 （TLR2） in distal ileum of rats with acute necrotizing pancreatitis（ANP） and its significance. METHODS： Sixty SD rats were randomly divided into ANP group （n = 40） and sham-operation group （n = 20）. TLR2 mRNA expression in distal ileum of rats was detected by real-time PCR, and its protein expression was determined by immunohistochemistry and Western blot. RESULTS： The expression levels of TLR2 mRNA and its protein were significantly higher in the ANP group than in the sham-operation group. Western blot showed that the TLR2 mRNA expression level was correlated with the pathological scores （r = 0.42, P 〈 0.01） and permeability （r = 0.41, P 〈 0.011） of intestinal mucosa. Immunohistochemistry displayed that the TLR2 protein expression was rather high on ileum mucosa surface, in T and B lymphocytes,histoleucocytes and eosinocytes of lamina propria, arteries and veins of submucosa, longitudinal and circular muscular layers of the ANP group. CONCLUSION： Expression of TLR2 is increased in the distal ileum of rats with ANP. Its overexpression may correlate with intestinal mucosa injury and development of enterogenic infection.