庚型肝炎病毒(GBV-C/HGV)与HBV和HCV联合或重叠感染的初步研究

PERMARILY STUDY ON COINFECTION OR SUPERINFECTION OF GBV-C/HGV WITH HEPATITIS B VIRUS(HBV)AND HEPATITIS C VIRUS(HCV)

目的研究庚型肝炎病毒(GBV-C/HGV)与 HBV 和 HCV 联合或重叠感染的情况,方法采用 ELISA 的方法检测血液制剂和血源乙肝疫苗原料血浆中的 HBsAg 和抗-HCV;并用5′非编码区的引物,用逆转录聚合酶链反应(RT-PCR)法检测 GBV-C/HGV RNA。结果 HBsAg 和抗 HCV 均阴性血浆的 GBV-C/HGV RNA 阳性率为18.8%(15/80);HBsAg 阳性而抗 HCV 阴性血浆的 GBV-C/HGV RNA 阳性率为29.4%(5/17);HBsAg 和抗 HCV 均阳性血浆的GBV-C/HGV RNA 阳性率为46.0%(29/63)。结论该供血人群中 GBV-C/HGV 与 HBV 和 HCV 联合或重叠感染率高于单独感染。

Objective To investigate the coinfection or superinfection of GBV-C/HGV with hepatitis B virus(HBV)and hepatitis C virus(HCV).Methods HBsAg、anti-HCV and GBV-C/HGV RNA were detected by ELISA and RT- PCR,respectively.Results Tphe results showed that 15 of 80(18.8%)with both HBsAg and anti-HCV negative,5 of 17(29.4%)with HBsAg positive and 29 of 63(46.0%)with both HBsAg and anti-HCV positive plasma samples were GBV-C/HGV positive.Conclusions The experimental data indicated that the coinfection or superinfection of GBV-C/HGV and HBV or HCV is more frequent than GBV-C/HGV infection alone.