心肌肌凝蛋白轻链-1的免疫组化研究

Immunohistochemical research of cardiac myosin light chain 1

为了研究心肌肌凝蛋白轻链－１（ＣＭＬＣ－１）组织特异性的特点，并对其构型在心肌发育过程中的表达规律以及不同哺乳动物ＣＭＬＣ－１构型的进化规律进行探讨，应用抗人心室肌ＣＭＬＣ－１单克隆抗体（ＭｃＡＢ）对成人和不同胎龄胎儿的心肌和其它组织、不同种属哺乳动物的心肌进行了免疫组织化学研究。结果发现：成人心室肌，慢性骨骼肌均发生强烈反应，成人心房，胎儿心室肌发生交叉反应，而其它组织如快型骨骼肌、平滑肌以及肝脾等非肌组织不发生反应。胎儿心肌的交叉反应程度随胎龄增加而增强，哺乳动物心肌均发生交叉反应，且随动物进化等级的提高呈增强趋势。结果说明：人ＣＭＬＣ－１具有一定的组织特异性，ＣＭＬＣ－１的表达与心脏发育和动物进化程度相关。

To investigate the issue specifical characterics of human cardiac myosin light chain 1 (CMLC 1),the changes in CMLC 1 isoforms expression during the development and the evolutionary relationships of the CMLC 1 isoforms in different species of mammalian animals, we examined CMLC 1 in adult and fetal human hearts and other tisssues and mammalian hearts immuohistochemically by using of the monoclonal antibody against human ventricular light chain 1 (MabVLC 1). The results showed the adult human ventricle and slow type skeletal muscles were strongly crossreacted with MabVLC 1, and the human adult atria and fetel ventricle were moderately crossreacted with MabVLC 1;and other human tissues including fast type skeletal muscle, smooth muscle and liver or spleen were not crossreacted. The degree of the fetal ventricle crossreacted with MabVLC 1 increaced with advance of the fetal ages. The heart tissues of all kinds of mammalains were crossreacted with MabVLC 1,and the reactivity increaced with the evolutionary degree of the mammlain species. The results suggest that (1) The CMLC 1 express specially in the ventricl and slow type muscles;(2)The expression of the CMLC 1 in ventricle is developmentally and evolutionaryly getting move.