人肝假性胆碱酯酶活性微量DTNB测定法

The micro DTNB colorimetric assay for human liver butyrylcholinesterase

ＤＴＮＢ（５，５－二巯基双－２－硝基苯甲酸）法测定乙酰胆碱酯酶活性由Ｅｌｍａｎ首创于１９６１年。与其它活性测定方法相比，ＤＴＮＢ测活性法具有操作快速简便、灵敏度高的优点，适于大量样本的检测，因此在科研工作及临床检验中被广为采用。本研究首次建立了人肝匀浆假性胆碱酯酶微量ＤＴＮＢ活性测定方法，并对人肝假性胆碱酯酶的酶促反应性质做了研究。

DTNB (Dithio Bis(2 Nitrobenzoic Acid)) method was first established for determination of acetylcholinesterase activity by Ellman in 1961. Comparing with other methods, DTNB method is more convenient, rapid and sensitive for a large number of samples assay. DTNB method has been widly applied in research and clinical practice. In the present study, A micro colorimetric assay was first established for determination of butyrylcholinesterase activity in human liver. The catalytic property of BChE in human liver homogenate was also studied.