原发性肺癌p53蛋白、增殖细胞核抗原表达及DNA含量与预后的关系

THE EXPRESSION OF p53 PROTEIN AND PCNA AND DNA CONTENTS IN THE PROGNOSIS OF LUNG CANCER

目的探讨ｐ５３蛋白、增殖细胞核抗原（ＰＣＮＡ）在肺癌中表达的特征、相关性及与ＤＮＡ含量和预后的关系。方法应用免疫组织化学方法检测原发性肺癌组织标本ｐ５３蛋白和ＰＣＮＡ的表达，并用图像分析技术测定ＤＮＡ含量。结果ｐ５３蛋白阳性表达组ＤＮＡ含量（８．９１Ｃ）和ＰＣＮＡ半定量分级（Ｉ～Ⅳ级例数为９，１３，１２，１０，下同）显著高于ｐ５３蛋白阴性组（６．６０ｃ）（１４，１５，７，２）在肺腺癌，半年内死亡组ｐ５３蛋白阳性表达率（１１／１５）和ＰＣＮＡ半定量分级（２，５，４，４）显著高于５年以上生存组（７／１９）（１０，６，１，２），且两者表达与淋巴结转移有显著关系；在肺鳞癌，半年内死亡组ＰＣＮＡ半定量分级（４，１，６，２）显著高于５年以上生存组（６，１４，７，０），而ｐ５３蛋白表达与患者预后关系不明显。结论ｐ５３蛋白、ＰＣＮＡ及ＤＮＡ含量间具有显著正相关关系，且ｐ５３蛋白、ＰＣＮＡ表达对于估测肺癌患者的预后有重要意义。

PURPOSE To study the relationship of p53 protein, PCNA and DNA contents to prognosis of lung cancer. METHODS We examined the expression of p53 protein and PCNA with histoimmunochemical technique,and DNA contents with image analysis technique in 82 cases of primary lung cancer. RESULTS DNA contents(8.91C)and PCNA semiquantitive grade(case of Ⅰ～Ⅳgrade was 9,13,12,0,respectively) of p53 positive cases was higher than that of p53 negative cases(6.60C)(14,15,7,2);in adenocarcinomas, p53 positive rate(11/15)and PCNA semiquantitive grade(2,5,4,4)in patients surviving for less half a year was higher than that in patients surviving for more than 5 year (7/19) (10,6,1,2),and p53 PCNA expression were all correlated with lymph node metastasis; in squamous cell carcinomas, PCNA semiquantitive grade (4,1,6,2)in patients surviving for less half a year was higher than that (6,14,7,0)in patients surviving for more than 5 years, but the expression of p53 was not. CONCLUSIONS There was significant correlation among p53 protein, PCNA and DNA contents;the detection of p53 and PCNA has great value in assesing the survival time of patients.