肺腺癌细胞核形态与预后关系的定量研究

QUANTITATIVE STUDY ON RELATIONSHIP BETWEEN NUCLEAR MORPHOMETRY AND PROGNOSIS IN LUNG ADENOCARCINOMA

目的探讨肺腺癌细胞核形态变化在不同临床病理条件下与预后之间的关系。方法应用多功能计算机图像分析仪选择８项参数（细胞核ＤＮＡ含量，核面积，核周长，最大、最小和等效直径，核轴比和核形状因子）对３４例术后生存时间有明差异的肺腺癌标本进行检测。结果淋巴结转移阴性、组织学Ⅰ、Ⅱ级和肿瘤长径＜５ｃｍ组，细胞核大者生存期短；淋巴结转移阳性、组织学Ⅲ级和肿瘤长径≥５ｃｍ组，细胞核大小与患者预后的关系不明显。生存组细胞核形状因子较大，且与各临床病理因素无明显相关。结论细胞核形态定量，尤其是细胞核形状因子对肺腺癌患者预后的评估有重要意义。

URPOSE To study the prognostic value of morphometry in lung adenocarcinoma with different clinical pathological features. METHODS Nuclear morphometry was performed with image analysis technique in 34 cases of lung adenocarcinoma which involved patients with long survival time and those with short survival time. RESULTS In the groups with no lymph node metastasis,histological grade Ⅰ,Ⅱ and diameter of tumor <5cm,the patients who died in half a year after surgery showed larger cancer cell nuclei than those surviving for more than 5 years ( P <0 05). In groups with lymph node metastasis, histological grade Ⅲ and diameter of tumor ≥5cm,there was no significant correlation between nuclear size and patient prognosis. In patients surviving for more than 5 years,the values of nuclear shape factors were larger and they had no significant relation to different clinical and pathological features. CONCLUSIONS Nuclear morphometry,especially nuclear shape factor,have important value in predicting prognosis of patients with lung adenocarcinoma.