内皮素等指标检测与Ⅱ型糖尿病并发症

Relationship between the Determination of Endothelin(ET) and Other Agents and the Complication of Diabetes Ⅱ

用放免分析（ＲＩＡ）法对３５例正常人和４７例Ⅱ型糖尿病人进行了内皮素（ＥＴ）水平检测；利用酶学终点法和免疫透射比浊法对ＴＧ、ＴＣ、ＨＤＬ－Ｃ、ＬＤＬ－Ｃ和ａｐｏＡＩ及ａｐｏＢ分别进行了测定，用酶联免疫吸附（ＥＬＩＳＡ）法测定了ａｐｏＥ水平。结果Ⅱ型糖尿病时ａｐｏＡＩ及ＨＤＬ－Ｃ均明显低于正常人（Ｐ＜０００１），其余指标均较正常人明显偏高（Ｐ＜００５或Ｐ＜０００１）；糖尿病控制好坏血浆ＥＴ－１水平在短期内变化不明显，但随糖尿病病情加重ＥＴ－１水平增高，高胰岛素与ＴＧ的变化在Ⅱ型糖尿病时呈显著正相关（Ｐ＜００５），多数指标在Ⅱ型糖尿病合并心血管疾病时的变化比未合并心血管疾病时的变化更显著。提示Ⅱ型糖尿病的治疗思路应当是控制血糖，降低胰岛素和血脂水平。

The concentration of the endothelin(ET); TG, TC, HDL-C, LDL-C, apoA1, apoB and apoE in 35 normal persons and 47 patients with diabetes Ⅱ was tested separately with radioimmunoassay(RIA); enzymology end point, immunotransparent nephelometry and enzyme linked immunosorbent assay(ELISA), to make a further study on the principles of diabetes Ⅱ combined with cardiovascular disease.The results showed that apoAI and HDL-C in diabets Ⅱ patients were clearly lower than that in normal persons ( P <0 001), but the other indexes were clearly higher ( P <0 05 or P <0 001), the control of diabetes mellitus in a short time could not affcet the ET-1 concentration, but with the condition becoming worse the ET-1 concentration would increase, the high insulin concentration and the change of TG were evidently correlated with diabetes Ⅱ ( P <0 05), the change of the most indexes in diabetes Ⅱ combined with cardiovascular disease were more evident than those without cardiovascular disease.It is suggested that the treatment of diabetes Ⅱ should begin with controling blood sugar, lowering insulin and blood lipid, increasing the ability of patients, antioxidation while reducing the damagement of endotheliocyte.These also are important measures of reducing complications and death rate.