中国对虾球状病毒垂直传播的初步研究

A Primary Study of the Vertical Transmission of a Kind of Spherovirus in Penaeus chinensis

应用透射电镜技术在越冬中国对虾雌性亲虾体内检测出一种球状病毒，直径为80nm左右。人工感染实验证实该病毒颗粒具有感染性。对带毒亲虾在室内隔离条件下进行了暂养催产，并对其所产的卵子、幼虫和的虾进行了隔离培育。带毒亲虾的卵子孵化率、幼虫成活率和幼虾生长速度均明显低于不带毒亲虾的子代。电镜观察发现．在带毒亲虾卵巢、各期幼虫和幼虾的细胞内存在球状病毒颗粒，在刚产的卵子的卵黄颗粒中可看到一种似球状病毒颗粒。研究表明：带球状病毒的对虾呈隐性感染，在胁迫条件下带毒幼虾可暴发病害，带毒亲虾可正常发育产卵，病毒可能通过卵巢、卵子进行垂直传播。

With electron microscope, a kind of spherovirus was observed in plasma of penaeid shrimp Puns Chinensis at different stages, such as eggs, larvae and juvenile shrimps. The artificial infection experiment verified that. the spheroviral particle has infectivity. The offsprings coming from carrying virus parental shrimp were infected generally in the separated breeding condition. A kind of spherovirus - like particle was found out in just spawned eggs. The result showed that the spherovirus probablly can be transmitted vertically.