用蚀斑法滴定痘苗病毒的准确性及其精确数学模型的探讨

Plaque Assay Method for Vaccinia Virus: Accuracy and Its Mathematical Model

用蚀斑法滴定病毒是确定感染病毒颗粒存在数量的一种较准确方法。本实验表明，痘苗病毒吸附4h后仍有大量病毒粒子未能吸附到细胞单层，进而测定出病毒接种量、维持液加量和所测病毒滴度间具有一种互为消长的非线性相关性。因而设计了几种检测方法，其准确性均优于常规痘苗病毒蚀斑测定法。利用装配有Mathematic软件包的计算机在痘苗病毒接种量、维持液加量和所测病毒滴度间建立了曲线拟合模型和曲面拟合模型。通过曲线拟合模型推断病毒感染滴度为常规法滴定值的近5倍。

Titrating infective virus by plaque assay is one of the most accurate methods which could determind the numbers of infective virus. The result shows that a considerable number of vaccinia viruses in inoculum were still unadsorbed to cv - 1 cell monolayer after a period of 4 hours adsorption time. A reciprocal and unlinear relationship between the titer of virus and the volume of inoculum, the volume of liquid medium was detected. According to this relationship, several new plaque assay methods, which had the advantage over routine method, were designed. By means of electronic computer equipped with mathematical software package, the curve fitting model and the surface fitting model of titer with the volume of virus inoculum and the volume of medium had been established. By curve fitting model, the extrapolated value of vaccinia virus titers was obtained five fold higher than the titers by routine method.