三维荧光二阶校正法用于血浆和尿液中柔红霉素的快速测定

Rapid Determination of Daunomycin Hydrochloride in Plasma and Urine Using Three-dimensional Fluorescence Coupled with Second-Order Calibration

文章采用三维激发发射荧光光谱与化学计量学交替三线性分解(ATLD)二阶校正法相结合,对血浆液和尿液中柔红霉素(DM)进行定量测定。实验不需对血浆和尿液预测样进行萃取等分离预处理,选取激发波长410～530nm,发射波长550～650nm,分别每隔5nm取一个数据,利用激发发射荧光扫描分别获得两个三维响应数阵(大小为21×25×12)。当组分数选择为3时,血浆和尿液校正集中盐酸柔红霉素的相对浓度与实际浓度的相关系数分别为r1=0.9990和r2=0.9952,经ATLD算法解析得到的血浆和尿液预测样中柔红霉素平均回收率分别为(92.8±7.6)%和(94.7±4.4)%。实验结果表明,此法能够解决血浆和尿液中盐酸柔红霉素药物因血浆和尿液内源物质与分析物光谱重叠所引起的难分辨的问题,可用于未知干扰共存下柔红霉素的直接快速定量测定。

A novel method was proposed for the rapid determination of Daunomycin Hydrochloride （DM） in plasma and urine by combining the excitation-emission fluorescence spectra with the second-oMer calibration based on the alternating trilinear decomposition algorithm. The method didn＇t need the separate pretreatment procedure such as extraction, and selected the excitation wavelengths from 410 to 530 nm and the emission at 550 to 650 nm and recorded at an interval of 5 nm, respectively. For the plasma and urine samples two three-way arrays of dimensions 21 ×25×12 were obtained. When their component numbers were set to 3, the line-arities were confirmed over the calibration range with correlation coefficients r1= 0.9990 and r2 = 0.9952 in plasma and urine, respectively. The obtained average recoveries of DM in plasma and urine were （92.8±7.6 ） % and （94.7 ± 4.4） % , respectively. It was shown that the proposed method could solve the problem of serious fluorescence spectral overlapping of the sought-for analyte （DM） and plasma or unine interference （s）, and it can be applied to rapid determination of DM even in the presence of unknown interference（s）.