超高效液相色谱-质谱法测定油脂中的10种抗氧化剂

Simultaneous Determination of 10 Antioxidants in Oils and Fats by Ultra Performance Liquid Chromatography-Mass Spectrometry

对油脂中的没食子酸丙酯(PG),没食子酸辛酯(OG)、2,4,5-三羟基苯丁酮(THBP)、叔丁基对苯二酚(TBHQ)、叔丁基对羟基茴香醚(BHA)、2,6-二叔丁基对甲酚(BHT)、没食子酸十二酯(DG)、正二氢愈创酸(NDGA)、2,6-二叔丁基-4-羟甲基苯酚(HMBP)和没食子酸异戊酯(IAG)10种抗氧化剂进行了超高效液相色谱-质谱法(UPLC/MS)测定的系统研究,建立了一种简单、快速、准确测定油脂中10种抗氧化剂的测定方法。对样品中提取溶剂和提取时间的选择以及相关线性、精密度、回收率作了考察和实验。结果表明,本方法能有效提取油脂样品中的这10种抗氧化剂,并对其进行UPLC/MS分离和测定。10种抗氧化剂的平均回收率为92.56%～102.5%(n=6),相对标准偏差为0.68%～5.12%(n=6),检出限为2～10μg/L。

A method was proposed for the determination of 10 antioxidants in oils and fats. Ultrasonic extraction was adopted for sample treatment. Extraction solutions were then analyzed directly by ultra performance liquid chromatography-mass spectrometry（UPLC/MS）. The method was simple, rapid, and could be used for the extraction, identifcation and quantification of 10 antioxidants in oils and fats. The recoveries（ n =6） were 92.56% -102.5%, the relative standard deviations（n=6） were 0.7% -5.1%, and the limits of detection were 2-10μg/L.