不同处理对草莓成花过程中钙·钙调素及同化物含量的影响

Effects of Different Treatments on the Contents of Calcium,Calmodulin and Its Assimilates in the Flower Formation Process of Strawberry

[目的]探讨不同促控剂对草莓成花过程中Ca2+-CaM及同化物含量变化的影响。[方法]以草莓品种宝交早生的当年生子苗为试材,用10 mmol/LEGTA、5 mmol/LTFP和2μmol/LA2318(7清水为对照),对草莓植株进行叶面喷洒试验。[结果]叶片中的Ca2+含量在花芽分化始期时有一积累高峰,花序分化期再次积累成峰值;而顶芽中CaM的含量高峰与叶片Ca2+峰值同时出现或稍后。A23187处理可使植株Ca2+和CaM在花芽分化始期的高峰提前;EGTA处理使植株的Ca2+的含量下降,但CaM的含量变动规律性不大;而TFP可降低植株的CaM含量,但对植株的Ca2+影响不大。各处理植株的可溶性糖、还原糖和淀粉含量在成花前均处于高水平。植株的蛋白质含量在花序原始体出现时形成峰值,随后缓慢降低。[结论]该研究为阐明Ca2+-CaM在草莓花芽分化中的作用机制提供依据。

[Objective] The research aimed to discuss the effects of different plant growth regulators on the contents changes of Ca^2＋-CaM and its assimilates in the flower formation process of strawberry. [Method] With the one-year-old seedlings of strawberry variety Fragaria ananassa Duch cv.Hokowase as tested materials, the leaves of strawberry plants were sprayed with 10 mmol/L EGTA, 5 mmol/L TFP and 2 μmol/L A23187（with clean water as CK） in the test. [Result] The content of Ca^2＋ in the leaves had an accumulation peak in initial stage of flower bud differentiation and another peak in the inflorescence differentiation stage. While the peak of CaM content in apical bud and Ca^2＋ content in leaf appeared at the same time or later. A23187 treatment could advance the peak of Ca^2＋ and CaM in the initial stage of flower bud differentiation. EGTA treatment reduced Ca^2＋ content in the plants, but the content of CaM changed little. TFP could reduce CaM content in the plants, but its effects on Ca^2＋ in the plants was not great. The contents of soluble sugar, reducing sugar and starch maintained at high level before the formation of flower. When the inflorescence primordium appeared, the protein content in the plants formed the peak value and then was slowly reduced. [Conclusion] The research provided basis for clarifying the mechanism of Ca^2＋-CaM in flower bud differentiation of strawberry.