甘薯品种南薯88的组织培养及植株再生研究

Study on the Tissue Culture and Plant Regeneration of Good Quality Sweet Potato Variety Nanshu 88

[目的]为南薯88的离体筛选及遗传转化奠定基础。[方法]以南薯88的叶片和叶柄为外植体,置于不同激素配比的培养基中,进行组织培养及植株再生,研究不同激素及外植体对南薯88植株再生的影响。[结果]单独使用6-BA、NAA诱导愈伤组织的效果不理想。过高浓度的2,4-D不利于外植体根的形成,也不利于芽的诱导。在MS+NAA1 mg/L+6-BA0.5 mg/L、MS+2,4-D0.05 mg/L+KT1 mg/L培养基中,两种外植体的出愈率均达100%,根分化率均达100%,叶片的芽分化率分别为20%、7%,叶柄的芽分化率均为7%,成功获得再生植株。相同的培养基,不同外植体的愈伤组织生长情况差异不大,器官的分化表现出一定的差异。[结论]南薯88组织培养较适宜的培养基为MS+NAA1 mg/L+6-BA0.5 mg/L、MS+2,4-D0.05 mg/L+KT1 mg/L。南薯88叶片的分化能力大于叶柄。

[Objective] The purpose of the study was to lay foundation for in vitro screening and genetic transformation of Nanshu 88. [Method] The leaf and leafstalk of Nanshu 88, as explants, were put into the media with different hormone combinations for tissue culture and plant regeneration so as to study the influences of different hormones and explants on the plant regeneration of Nanshu 88. [Result] The effect of solely using 6-BA and NAA to induce callus was not perfect. 2,4-D with too high concentration was not favorable for the root formation and bud inducement of explants. On the media of MS＋NAA 1mg/L＋6-BA 0.5 mg/L and MS＋2,4-D 0.05 mg/L＋KT 1mg/L, the callus induction and root differentiation of the 2 explants all achieved 100%, the bud differentiation rates of leaf were 20% and 7% resp., the bud differentiation rates of leafstalk were all 7% and the regenerated plants were obtained successfully. On the same media, the growth conditions of calli from different explants had little difference and the differentiations of organs showed some difference. [Conclusion] The more suitable media for tissue culture of Nanshu 88 were MS＋NAA 1 mg/L＋6-BA 0.5mg/L and MS＋2,4-D 0.05 mg/L＋KT 1 mg/L. The differentiation ability of Nanshu 88 leaf was bigger than that of leafstalk.