老鹰茶中黄酮类化学成分分析

Analyse on the chemical constituents from flavonoids of Litsea coreana L

目的分析老鹰茶总黄酮的化学组成,为老鹰茶这一药用资源的进一步开发利用提供科学依据。方法采用聚酰胺柱色谱、硅胶柱色谱、sephadex LH-20柱色谱等柱层析的方法分离提纯,通过紫外分光光度法(UV)、核磁共振氢谱(1H-NMR)、核磁共振碳谱(13C-NMR)、质谱(MS)等波谱方法及化学方法鉴定它们的化学结构。结果分离得到4个黄酮苷类化合物,经光谱学方法鉴定它们的化学结构,分别为:槲皮素-3-O-β-D-半乳糖苷(Ⅰ)、山柰酚-3-O-β-D-葡萄糖苷(Ⅱ)、槲皮素-3-O-β-D-葡萄糖苷(Ⅲ)、山柰酚-3-O-β-D-半乳糖苷(Ⅳ)。结论化合物Ⅰ和Ⅳ为首次从该植物中分离得到。

Objective To analyse chemical constituents from general flavonoids of Lietsea coreana L, provide evidences for exploitation of Lietsea coreana L. Methods The constituents were separated and purified repeatedly by methods of silica gel column chromatography, polyamide column chromatography and sephadex LH-20 column chromatography and their structures were identified by ^1 H-NMR, ^13C-NMR, MS and chemical methods. Results Four flavonoids were isolated from Lietsea coreana L. Their structures were elucidated as quercetin-3-o-β-D-galactopyranoside（ Ⅰ ） kaempferol-3-o-β-D-glucopyranoside（ Ⅱ）, quercetin-3-o-β-D-glucopyranoside（ Ⅲ） and kaempferol- 3-o-β-D-galactopyranoside （ Ⅳ ）. Conclusion The compounds Ⅰ and Ⅳ are obtained from the plant for the first time.