中药新复方愈肠宁的组方研究

A Study on Components of Yuchangning as A New Herbal Recipes

目的筛选中药新复方愈肠宁的有效组分配伍。方法采用三硝基苯磺酸大鼠溃疡性结肠炎(ulcerative colitis,UC)模型对愈肠宁处方进行拆方及最佳配比的优选实验;采用两种UC模型大鼠对饮片配伍和有效组分配伍的愈肠宁药效进行比较。结果初步确定愈肠宁药味组成为:黄芪、苦参各15 g;拆方实验表明,二药合用疗效优于单用,且以黄芪:苦参为1:1时,疗效最佳;饮片配伍与有效组分配伍的疗效无明显差异,证实黄芪皂苷、黄芪多糖、苦参碱、氧化苦参碱为该方起效的物质基础,并确定了愈肠宁日服处方量为:黄芪多糖239 mg,氧化苦参碱150 mg,苦参碱50 mg,黄芪皂苷25 mg。结论筛选出以有效组分配伍的中药新复方愈肠宁,为中药新复方的开发提供了新思路。

Objective To filtrate a new herbal medicine recipe of yuchangning, which composed by the effective ingredients for treating UC,and explore a new research idea to develop herbal medicines. Methods UC model rats led by TNBS were treated with variant proportions contained radix astragali and raix sophorae flavescentis and the most prefered recipe was filtrated . And then to evalulate the effect difference between the common recipe composed of medicinal marerials and the most prefered recipe. Results Based on preliminary research results, the most preferred prescription was radix astragali 15 g, raix sophorae flavescentis 15 g. Moreover, we found that the curative effect of two herbs combination was better than that of anyone and especially, 1：1 rate of radix astragali and raix sophorae flavescentis showed a most strong curative effect. The curative effect between the prescription composed of common medicinal materials and the the most prefered prescription were similar, suggesting that astragalus saponin, astragalus polysaccharide, matrine and oxymatrine possiblely were effective components in this prescription. The prescription composed of astragalus saponin 25 mg, astragalus polysaccharide 239 mg, matrine 50 mg and oxymatrine 150 mg each day was reasonable. Conclusions A novel herbal medicine recipe. yuchangning , which composed of effective components, was invented and this provided a logical strategy for developing new herbal medicine recipes.