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缬沙坦对2型糖尿病模型大鼠肾组织巨噬细胞浸润的影响 被引量：5

Effect of Valsartan on Recruitment of Monocyte/Macrophage Cells in Renal Tissue of Type 2 Diabetic Rats
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摘要 目的探讨缬沙坦对糖尿病模型大鼠肾组织单核/巨噬细胞(MO/Mφ)浸润的影响。方法应用高糖高脂饮食加小剂量链脲佐菌素(streptozotocin,STZ)诱导建立单侧肾切除2型糖尿病模型大鼠,随机分为:单切对照组、糖尿病模型组、缬沙坦治疗组各10只,8周末检测各组大鼠尿清蛋白排泄率(UAER)、血糖(BG)、血肌酐(Scr)、血尿素氮(BUN)、内生肌酐清除率(Ccr)等变化;应用免疫组化技术检测各组肾组织中单核细胞趋化蛋白-1(MCP-1)与CD68的表达。结果缬沙坦治疗组大鼠肾重/体重、Scr、BUN、UAER明显低于模型组(P<0.05);模型组肾组织MCP-1的表达及CD68阳性细胞数明显高于对照组(P<0.01);缬沙坦治疗组可明显下调MCP-1的表达,减少CD68阳性细胞浸润(P<0.01)。结论缬沙坦对糖尿病模型大鼠肾脏有明显保护作用,其机制可能部分与抑制肾组织MO/Mφ浸润有关。 Objective To explore the effect of valsartan on recruitment of monocyte/macrophage cells in renal tissue of type 2 diabetic rats. Methods Streptozotocin induced diabetic rats were randomly assigned to three groups： control group, diabetic model group and diabetic model with valsartan treatment group （10 mg·kg^-1·d^-1）. After 8 weeks, the expression of MCP-1 and CD68 in the renal tissue of type 2 diabetic rats was detected by immunohistoehemistry. In addition, BG, Scr, BUN, Ccr and UAER were measured respectively. Results Compared to diabetic model group, the level of UAER, S, cr, BUN, kidney/weigh in valsartan treated group were significantly decreased（P〈0.05）. The expression of MCP-1 and CD68 in the renal tissue of type 2 diabetic rats were increased in diabetic model group than that in control group （P〈0.01）. Valsartan could significantly down-regulate the expression of MCP-I and CD68 in the renal tissue of diabetic rats （P〈0.05）. Conclusion Valsartan was able to ameliorate the renal lesion in type 2 diabetic rats, through a potential mechanism partly underlain the inhibition of recruitment of monocyte/macrophage ceils in renal tissue of type 2 diabetic rats.
作者 吴泽成 刘慎微
机构地区 华中科技大学同济医学院附属同济医院肾内科
出处 《医药导报》 CAS 2008年第3期268-271,共4页 Herald of Medicine
关键词 缬沙坦 糖尿病肾病 单核细胞趋化因子-1 Valsartan Diabetes mellitus Monocyte chemoattractant protein-1
分类号 R972.4 [医药卫生—药品] R965.1 [医药卫生—药理学]
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参考文献10

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共引文献6

同被引文献46

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引证文献5

二级引证文献23

医药导报
2008年 第3期

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