柴胡总苷高效液相色谱指纹图谱与主成分含量测定

An Analysis of HPLC Chromatography Fingerprinting and Main Components of Total Saponin of Bupleurum Chinense DC.

目的研究柴胡总苷的高效液相色谱指纹图谱,测定柴胡总苷中柴胡苷a、d的含量。方法利用HPLC-DAD检测、梯度洗脱的方法,在210nm波长的条件下,测定了10批柴胡总皂苷样品。色谱条件：Hypersil C18柱（4.6 mm×250 mm,5μm）；流动相为乙腈A-水B,二元梯度洗脱：30%-40%A（0-20 min）,40%-50%A（20-35 min）,50%-60%A（35-45 min）；检测波长：210 nm；流速：1.0 mL·min^-1；柱温25℃；进样体积为10μL。结果10批柴胡总苷色谱指纹图谱具有很高的相似度,在相同色谱条件下测定了不同批次提取的柴胡总苷中柴胡苷a、d含量。表明柴胡总皂苷制备工艺稳定性良好,可有效地富集皂苷类化合物。结论柴胡总苷的指纹图谱特征性及专属性强,可为柴胡总苷的质量控制以及制备工艺的评价提供依据,再结合含量测定用于全面控制柴胡总苷的质量,确保每批产品的均一性。

Objective To map HPLC chromatography fingerprinting and measure the level of sarponin a＆d of total sarponin from bupleurum Chinense DC, Methods Using a Hypersil C18 column （4.6mm×250mm, 5μm）, a high performance liquid chromatography assay with gradient elution was developed to identify 10 samples at wavelength 210 nm. The chromatography conditions follow as ：the column temperature was set up at 25℃. the samplesize was 10 μL at 0.7 mL·min^-1 flow rate and a gradient solution of acetonitrile and water was consisted of 30%-40% acetonitrile （0-20 min ） , 40%-50% acetonitrile（20-35 min） and 50%-60% acetonitrile（35-45 min）. Results The chromatography fingerprinting from all of the 10 samples share a high similarity. With respect of the preparing process of the total saponin from bupleurum chinense DC, the validity and stability were significantly excellent. Conclusion The method established in the present study is convenient, reliable, and could be used for the quality control of the extract of the total saponin from bupleurum chinense DC and for the monitor and control of the preparation process. Based on this method, a higher quality of total saponin would be assure with strongly consistency in each group productions.