大蒜膏贴敷神阙穴整复睡眠剥夺所致昼夜节律紊乱的研究

Study of Adjusting Circadian Dysrhythmia Due to Sleep Deprivation by Point Shenque Application of Garlic Paste

目的观察神阙穴贴敷大蒜膏对抗睡眠剥夺所致昼夜节律紊乱的整复作用,初步探讨大蒜膏整复昼夜节律的作用机制。方法将南方医科大学20名健康成年男性随机分为三组,正常对照组(A)、睡眠剥夺组(B)、睡眠剥夺并贴药组(C),对B组和C组同时进行48 h睡眠剥夺,A组不做任何处理,观察A组及睡眠剥夺后B组和C组的血清单胺类神经递质(去甲肾上腺素和5-羟色胺)的浓度变化。结果三组的去甲肾上腺素(NE)均呈典型的昼夜节律性(A组和C组P<0.01,B组P<0.05)。A组峰值为158.377,大约出现在10:56;B组峰值291.529,出现在19:44左右;C组峰值255.964,出现在17:06左右。B组与A组相比峰相位出现了明显的偏移,C组的峰相位较B组有所恢复。A组的5-羟色胺(5-HT)有典型的近似昼夜节律性(P<0.01),B组和C组的节律消失(P>0.05)。A组峰值为196.563,大约出现在13:10。结论大蒜膏穴位贴敷可以调整人体紊乱的昼夜节律,在一定程度上促进紊乱的节律的恢复,是一种简便有效的抗昼夜节律紊乱的方法。

Objective To investigate the adjusting effect of point Shenque（ CV 8） application of garlic paste on circadian dysrhythmia due to sleep deprivation and preliminarily explore the mechanism by which it adjusts circadian rhythm. Methods Twenty healthy male adults from Nanfang Medical University were randomly allocated to three groups： normal control （A）, sleep deprivation （B） and sleep deprivation plus point application （C）. Forty-eight hour sleep deprivation was performed in group B and C at the same time. Group A was not treated. Serum monoamine neurotransmltter （ norepinephrine and 5-hydroxytryptamine） concentration was measured in group A, and group B and C after sleep deprivation. Results Norepinephrine （NE） had a typical circadian change in all the three groups （P〈0.01 in group A and C, P〈0.05 in group B）. The peak value was 158.377 and appeared at about 10：56 in group A, was 291. 529 and appeared at about 19：44 in group B, and was 255. 964 and appeared at about 17：06 in group C. The peak phase ob- viously deviated in group B as compared with group A and was somewhat restored in group C as compared with group B. 5- hydroxytryptamine （5-HT） had a typical approximate circadian rhythm in group A （P 〈 0.01 ）. Its circadian rhythm disappeared in circadian rhythm （P 〉0.05）. The peak value was 196.563 and appeared at about 13,：10 in group A. Conclusion Point application of garlic paste can adjust body＇ s circadian dysrhythmia and helps to restore the disordered rhythm to a certain extent. It is a simple and effective way to treat circadian dysrhythmia.