缺氧对大鼠中性粒细胞趋化能力的影响

Effect of hypoxia on chemotaxis of rat polymorphonuclear leukocytes

目的:研究急、慢性缺氧对大鼠中性粒细胞(PMNs)趋化能力的影响。方法:应用细胞微管吸吮实验技术,从单细胞角度研究在血清趋化作用下不同缺氧状态PMNs伪足生长的变化。结果:自身血清作趋化剂,急性、慢性缺氧组PMNs伪足长度分别为(1.200±0.178)μm和(1.094±0.164)μm,显著大于常氧对照组(0.914±0.156)μm(P<0.05);常氧、急性缺氧和慢性缺氧血清作用于常氧PMNs时,急、慢性缺氧血清作用下PMNs伪足长度分别为(1.059±0.179)μm和(1.041±0.175)μm,显著大于常氧血清作用(P<0.05);常氧血清作用于常氧、急性和慢性缺氧PMNs时,急性、慢性缺氧PMNs伪足长度分别为(1.058±0.163)μm和(1.118±0.126)μm,显著大于常氧对照组(P<0.05)。结论:急、慢性缺氧促进了大鼠PMNs趋化能力,缺氧后血液微环境的变化和PMNs响应能力的变化是其重要原因。

AIM： To explore the effect of acute and chronic hypoxia on the chemotaxis of polymorphonuclear leukocytes （PMNs） of rats. METHODS： Serum and PMNs were separated from rats under normoxia, acute hypoxia and chronic hypoxia condition, and cell aspiration technology system was used to determine the chemotactic migration of pseudo- pod of PMNs induced by serum as chemotactic factor. RESULTS： Induced by autoserum , the length of pseudopod of PMNs migrated into micropipette [ （ 1. 200 ± 0. 178） μm , （ 1. 094 ± 0. 164 ） μm ] under acute hypoxic and chronic hypoxic condition was significantly longer than the corresponding value for control[ （0. 914 ± 0. 156）μm, P 〈 0. 05 ] ; Using acute hypoxic serum＇and chronic hypoxic serum as chemotactic factor, the length of pseudopod of normoxic PMNs [ （ 1. 059 ± 0. 179）μm, （1. 041 ± 0. 175 ）μm ] was obviously longer than that induced by normoxic serum[ （0. 914 ± 0. 156 ）μm, P 〈 0. 05 ] ; Induced by normoxic serum, the length of pseudopod of acute hypoxic PMNs and chronic hypoxic PMNs [ （ 1. 058 ± 0. 163）μm, （1. 118 ± 0. 126）μm] was obviously longer than that of normoxic PMNs[ （0. 914 ± 0. 156）μm, P 〈 0. 05 ]. CONCLUSION： These data suggest that hypoxia might promote PMNs chemotaxis and migration, the changes of composition in blood microenvironment and the changes of response of PMNs to chemokines might be the intrinsic factors of PMNs chemotaxis changes after hypoxia.