Cyr61与β-catenin在肝癌组织和细胞株中表达的相关性研究

Relationship of Cyr61 and β-catenin in hepatocellular carcinoma tissues and hepatoma carcinoma cells

目的研究Cyr61与β-catenin在肝细胞肝癌和肝癌细胞株中的表达及其相关性,探讨二者在肝癌发生中的作用。方法采用免疫组化法检测32例肝细胞肝癌患者肝癌组织中Cyr61与β-catenin蛋白的表达水平;免疫组化法和RT-PCR检测正常肝细胞株L02和肝癌细胞株HepG2、QGY-7701、H22中Cyr61与β-catenin蛋白与mRNA的表达水平;并比较二者的相关性。结果65.63%(21/32)肝癌标本Cyr61蛋白阳性表达,71.88%(23/32)肝癌标本β-catenin蛋白阳性表达,二者合并阳性表达有56.25%(18/32),合并阴性表达有18.75%(6/32),Cyr61表达与β-catenin表达呈正相关(r=0·425,P<0·05);正常肝细胞株L02及肝癌细胞株HepG2、QGY-7701、H22中Cyr61与β-catenin mRNA与蛋白均有表达,二者表达一致性达100%。结论Cyr61与β-catenin在肝癌和肝癌细胞株中高度一致阳性表达,提示Cyr61的表达可能与β-catenin的表达有关,二者可能在肝癌的发生中起到重要作用。

Objective To investigate expressions of Cyr61 and β-catenin and their relationship in hepatocellular carcinoma tissues and hepatoma carcinoma cells, and explore their roles in hepatocarcinogenesis. Methods Expressions of Cyr61 and β-catenin were studied by immunohistochemistry in 32 patients with hepatocellular carcinoma, hepatocyte L02 and hepatoma carcinoma cell HepG2, QGY-7701 and H22 cells. Cyr61 mRNA and β-catenin mRNA were detected by RT-PCR in hepatocyte L02 and hepatoma carcinoma cell HepG2, QGY-7701 and H22 cells. The relationship of their expressions were studied. Results The expressions of Cyr61 protein and β-catenin protein were found in 65.63% （21/32） and 71.88% （23/32）of 32 patients, both Cyr61 protein and β-catenin protein were negatively expressed detected in 18.75% （6/32） and positively in 56.25% （18/32） respectively. Expression of Cyr61 protein was positively correlated with the expression of β- eatenin protein （r =0. 425, P 〈0. 05 ）. Expressions of Cyr61 and β-catenin at both mRNA and protein levels were found in hepatocyte 1.02 and hepatoma carcinoma cell HepG2, QGY-7701, H22, co-expression of Cyr61 and β-catenin was 100% of all ceils. Conclusion Cyr61 and β-catenin are expressed in hepatoceilular carcinoma tissues and hepatoma carcinoma ceils. Expression of Cyr61 is related with expression of β-catenin, and they may participate in hepatocarcinogenesis.