不同剪切力对豚鼠微血管内皮细胞形态学的影响

Effects of shear stress on morphology of different microvascular endothelial cells

目的观察不同剪切力对体外培养的豚鼠耳蜗、脑微血管内皮细胞的影响。方法用自制的流体力学装置在不同时相点、以不同剪切力对豚鼠脑、耳蜗微血管内皮细胞进行力学作用,获得形态学图像并进行形状参数Pyx的测定分析。结果通过胶原酶消化法可获得单层培养的豚鼠耳蜗、脑微血管内皮细胞。对于耳蜗微血管内皮细胞,剪切力为0·883dyn/cm2时作用24h未发生细胞形态改变;当剪切力增加到1.184dyn/cm2时,作用8h后细胞形态即开始出现顺流体方向的顺应性变化,随时间延长,变化趋势更明显。而脑微血管内皮细胞在剪切力0.267dyn/cm2时作用24h未发生细胞形态改变;当剪切力增加到1.069dyn/cm2时,作用4h后细胞形态即开始出现顺流体方向的顺应性变化。结论耳蜗、脑微血管内皮细胞在受到剪切力作用后其形态学不同于静态培养的内皮细胞。

Objective To observe the effects of different shear stresses on the morphological changes of cochlear and brain microvascular endothelial cells from guinea pigs, Methods We obtained the morphological photos of cochlear and brain microvascular endothelial monolayer cells with the hydrodynamic system we designed and detected the morphological parameters such as Pyx. Results After digestion with collagenase type I, we obtained the monolayer cells of cochlear and brain microvascular endothelial monolayer. As to cochlear microvascular endothelial cells isolated from guinea pigs, no morphological changes were found when shear stress was 0.883 dyn/cm^2 and the acting time was 24 h. When shear stress was 1. 184 dyn/cm^2 and the acting time was 8 h, compliant changes from former disorderliness into orderliness following the direction of liquid flowing in the cells occurred. This changing tendency was in a time-dependent manner. As to brain microvascular endothelial cells, no morphological changes were found when shear stress was 0.267 dyn/cm^2 and the acting time was 24 h. When shear stress was 1. 069 dyn/cm^2 and the acting time was 4 h, compliant changes began from former disorderliness into orderliness following the direction of liquid flowing in the cells. Conclusion Cochlear and brain microvascular endothelial cells from guinea pigs cultured under the condition of shear stress are morphologically different from statically cultured endothelial cells. Range of shear stress that two kinds of microvascular endothelial cells can tolerate is different.