摘要
研究了不同浓度NaCl胁迫对骆驼蓬幼苗Na+、K+含量及液胞膜H+-ATP酶(H+-ATPase)和H+-焦磷酸酶(H+-PPase)活性的影响。结果表明,NaCl胁迫浓度的增加使骆驼蓬幼苗根、叶中的K+含量下降,Na+含量和Na+/K+比及K+对Na+的吸收和运输选择性增高;根、叶液胞膜H+-ATPase和H+-PPase活性升高,H+-ATP酶耦联比率无显著变化;根液胞膜依赖ATP和PPi的质子泵活性及Na+/H+逆向转运活性先升后降,叶依赖ATP的质子泵活性及Na+/H+逆向转运活性升高,依赖PPi的质子泵活性先升后降。说明液泡膜H+-ATPase和H+-PPase驱动的质子泵和Na+/H+逆向转运活性对Na+在液泡内的积累及其骆驼蓬的耐盐性起重要作用。
Effects of the different concentration of NaCl stress on Na+ and K^+ contents,tonoplast H^+-ATPase and H^+-PPase activities in peganum harmala seedling leaves were studied.The results showed that with NaCl stress concentration increased,K^+ content in roots and leaves was decreased,but Na^+ content,Na^+/K^+ ratio,K^+/Na^+ selectivity,as well as tonoplast H^+-ATPase and H^+-PPase activities were all increased,the H^+/substrate stoichiometry was significantly unchanged,the activities of the ATP-dependent proton pump and the Na^+/H^+ antiport in leaves were increased,the activities of PPi-dependent proton pump in leaves and ATP and PPi-dependent proton pump and Na^+/H^+ antiport of the tonolast vesicles in roots were all increased and then decreased with the increase of NaCl concentration.These results indicated that the activity of Na^+/H^+ antiport and proton pump driven by H^+-ATPase and H^+-PPase in tonoplast of P.harmala plays an important role in Na^+ accumulation in vacuoles and salt tolerance.
出处
《中国沙漠》
CSCD
北大核心
2008年第2期274-279,共6页
Journal of Desert Research
基金
甘肃省教育厅科研基金项目(JY0510-07)资助