结缕草属植物种间关系的SSR分析

SSR Analysis on Inter-species Relationships in Zoysia Willd.

以结缕草属的9种1变种共58份种源为材料,利用SSR标记技术进行了多样性分析和种间关系研究。结果表明:结缕草属植物具有很高的遗传多样性;14对SSR引物中,共扩增出267条带,Nei’s基因多样性指数为0.376 0,Shannon多样性指数为0.552 6;属内总基因多样性为0.382 3,种内基因多样性为0.219 6,57.44%的遗传变异存在于种内,基因流为0.674 8,不同的种之间有遗传分化,遗传漂变对该遗传分化有一定的贡献。UPGMA遗传距离聚类分析结果表明,结缕草、中华结缕草、朝鲜结缕草和大穗结缕草聚为一类;沟叶结缕草、小结缕草和细叶结缕草聚为一类;大花结缕草、长花结缕草和太平洋结缕草各自为一类。研究表明,生态地理条件相似的种优先聚集。

The genetic diversity and relationship of 58 accessions of Zoysia Willd.belonging to 9 species and 1 variety were analyzed using SSR markers.267 bands were amplified from 9 species and 1 variety by 14 pairs reliable SSR primers.A high level of genetic diversity was revealed.The Nei＇s gene diversity index was 0.376 0 and the Shannon diversity index was 0.552 6.The total gene diversity was 0.382 3,while the gene diversity within species was 0.219 6,57.44% of genetic variation existed within species.The gene flow was 0.674 8,there was differentiation among species,the genetic drift cause genetic differentiation.The UPGMA dendrogram indicated that Z.japonica was clustered together with Z.sinica,Z.macrostachya and Z.koreana;Z.matrella was clustered together with Z.tenuifolia and Z.minima;Z.macrantha,Z.pacifica and Z.sinica var.longiflora was a group by itself respectively.Species with similar ecological and geographical conditions clustered first.Finally,the classification position of Z.sinica var.longiflora was discussed.